Precision Mutant Library
Traditional methods for library construction, including error-prone PCR or degenerate methods, suffer from limited control over codon usage and poor variant representation. This requires multiple rounds of screening to capture the entire variation of the library. CustomArray’s Precision Mutant Library technology offers precise control over each synthesized variant. The result is a more diverse and fully covered mutant library with unbiased distribution, ultimately saving valuable screening time, speeding up discovery workflow and reducing overall downstream expenses.
Key Benefits of Precision Mutant Libraries
Precision Control of user-defined condon usage.
Guaranteed >90% coverage of all desired variants.
Superior library diversity with library size > 10 to the 10th power
Using Next-Generation Semiconductor Technology for Library Construction
A combinatorial mutagenesis library for 4 sequential amino acid positions was created using either degenerate (NNK) condons or using our precise oligos. Specific amino acids were requested at each site.
Using our semiconductor-based oligos in library construction:
Eliminates the significant condon bias incurred with NNK degenerate oligos
Achieves user-defined amino acid distribution with equal representation of variants
Each bar represents a mutated position and each color represents the frequency of variants with a particular amino acid.
A well-constructed mutant library can be a very useful tool for a variety of protein engineering projects, including:
Improving antibody binding affinity through CDR engineering
Optimizing enzyme affinity and stability
Identifying critical residues within a protein domain
Improving ligand-receptor binding
Antibody affinity maturation
Optimizing protein structure